Mettl5 as an 18S rRNA m6A methyltransferase interacts with ribosome proteins. (A). Schematic diagram of Mettl5 knockout in mESCs. (B). RNA-Seq tracks of Mettl5 mRNA. Deleted Mettl5 exons are indicated with red box. (C). Distribution of m6A targets in control mESCs. (D). Distribution of m6A targets in Mettl5 KO mESCs. (E). Sequence motif identified in m6A MeRIP-seq. The upper is m6A peaks of control mESCs and the bottom is m6A peaks of Mettl5 KO mESCs. (F). m6A MeRIP-Seq tracks of 18s rRNA (Rn18s, chr17:39846354-39848202) within 45s rRNA (Rn45s, chr17:39,840,997-39,850,829). Decreased 18S rRNA peaks are indicated with red highlight. (G). SDS-PAGE of METTL5 protein complex. (H). String view of METTL5 interacting proteins.