TABLE 2.

Mutations of the rpoB gene found in Rif^r M. tuberculosis isolates from Brazil^ad

Mutated codon	Specific mutation	No. (%) of mutated sites/origin of isolate
511	CTG(Leu)→CCG(Pro)	1 (1.2)/RS
513	CAA(Gln)→CCA(Pro)	1 (1.2)/RJ
516	GAC(Asp)→GTC(Val)	5 (6.2)/RS and RJ
	TAC(Tyr)	1 (1.2)/RS
522	TCG(Ser)→TTG(Leu)	2 (2.4)/RS
526	CAC(His)→CTC(Leu)	2 (2.4)/RS
	TAC(Tyr)	9 (11)/RS
	CGC(Arg)	2 (2.4)/RS and SP
	TGC(Cys)c	2 (2.4)/SP
	GAC(Asp)	1 (1.2)/SP
	CTG(Leu)bc	1 (1.2)/SP
531	TCG(Ser)→TTG(Leu)	41 (50)/RS, SP, and RJ
	TGG(Trp)	3 (3.7)/RS
533	CTG(Leu)→CCT(Pro)bc	1 (1.2)/SP
531 and 514	TCG(Ser)→TTG(Leu) and TTC(Phe)→GTC(Val)b	1 (1.2)/RJ
531 and 526	TCG(Ser)→TTG(Leu) and CAC(His)→TGC(Cys)c	1 (1.2)/RJ
511 and 516	CTG(Leu)→CGG(Arg) and GAC(Asp)→GTC(Val)	1 (1.2)/RJ
511 and 515	CTG(Leu)→CCG(Pro) and ATG(Met)→ATA(Ile)	1 (1.2)/RJ
524, 525, 526, and del 527	TTG(Leu)→TGG(Trp), ACC(Thr)→CCC(Pro), CAC(His)→CAG(Gln), and del AAG(Lys)b	1 (1.2)/RJ
513 and del 514, 515, and 516	CAA(Gln)→CAC(His) and del TTC(Phe), ATG(Met), and GAC(Asp)b	1 (1.2)/RJ
Del 515, 516, 517, and 518	Del ATG(Met), GAC(Asp), CAG(Gln), and AAC(Asn)b	1 (1.2)/RJ

^aCodon numbers correspond to the Escherichia coli numbering system for the RNA polymerase β subunit. The mutated bases are shown in bold. Del, deletion of. 

^bNew allele. 

^cDouble mutations in the same codon. 

^dThe Rif^r isolates showing no mutation within the 69-bp hypervariable region (3 of 82) were not included.