Figure 3.

DE circRNAs candidates were validated by RT-PCR between SHR and WKY rat aorta. (A) The seven DE circRNAs were detected by the RT-PCR between SHR and WKY rat aorta. Five significant aortic circRNAs were confirmed in hypertensive rats, including rno_circRNA_0005818, rno_circRNA_0005304, rno_circRNA_0005506, rno_circRNA_0009301, and rno_circRNA_0009197. (B) The EVG staining illustrated that the regulation of circRNAs were associated with thin elastic fiber and thick collagen fiber. Black color represents elastic fiber, red color represents collagen fiber. n = 10 for each group, ***P < 0.001. DE, differentially expressed; circRNAs, circular RNAs; RT-PCR, real-time polymerase chain reaction; WKY, Wistar-Kyoto; SHR spontaneously hypertensive rat.