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. 2021 Dec 20;9:793389. doi: 10.3389/fcell.2021.793389

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Copyright © 2021 Ge, Zhang, Tang, Hu, Hou, Sun, Han and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PDK1 overexpression increases the phosphorylation level of Ser232-PDHE1α in diabetic oocytes. (A) Diagram showing the experimental protocol to examine the effects of PDK1 overexpression on meiotic apparatus in diabetic oocytes. PBS or exogenous Myc-Pdk1 mRNA was microinjected into GV oocytes. (B) Efficiency of PDK1 overexpression (PDK1-OE) after mRNA injection was confirmed by immunoblotting, probing with anti-MYC antibody. (C) Control, diabetic, and PDK1-overexpressing diabetic oocytes were stained with pSer232-PDHE1α antibody (green) and with PI for chromosomes (red). (D) Quantification of pSer232-PDHE1α fluorescence show in (C). Scale bar: 20 µm. Error bars indicate ± SD, a Student’s t test was used for statistical analysis.