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. 2021 Dec 20;9:769213. doi: 10.3389/fcell.2021.769213

FIGURE 7.

FIGURE 7

Role of Mfn2 in PERK signaling pathway related-mitochondrial dysfunction, MAMs reduction and apoptosis. (A–D) Representative Western blots of Myc-tag, p-PERK, PERK, cleaved caspase3, CHOP, Mfn2 expression and quantitation per group (n = 3). (E, F) Representative images of MMP in cultured podocytes by JC-1 staining and quantification per group (n = 3) (original magnification, ×200). (G,H) Representative images of DCFH-DA fluorescence (green) in cultured podocytes to assess ROS production and quantification per group (n = 3) (original magnification, ×100). (I) ATP production was assessed and quantification per group (n = 3). (J, K) Flow cytometry analysis of apoptosis in cultured podocytes and quantitation per group (n = 3). 1, 2, 3, 4, 5, 6 represent N, Scramble, shRNA Mfn2, HG, HG + pcDNA3.1, HG + Mfn2-Myc, respectively; N = 5 mM glucose for 24 h; HG = 25 mM glucose for 24 h; Scramble = shRNA-scrambles; shRNA = short hairpin RNA; *p < 0.05 compared with podocytes cultured in normal conditions; #p < 0.05 compared with podocytes treated with HG.