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. 2021 Dec 20;12:780092. doi: 10.3389/fmicb.2021.780092

FIGURE 1.

FIGURE 1

Experimental design of in vitro continuous PolyFermS fermentations inoculated with immobilized chicken cecal microbiota. Single cecal microbiota was immobilized in a gellan–xanthan gel for each fermentation. (A,B) The immobilized cecal microbiota of chicken 1 was used to inoculate two bioreactors (30% v/v); F1-A and F1-B and continuously fed with mVL-1 and mVL-2 fermentation medium, respectively. (C) F2 consisted of an inoculum reactor (IR) containing cecal beads of chicken 2, connected to four second-stage reactors (SSR) and continuously fed with 5% fermentation effluent of IR and 95% fresh fermentation mVL-3 medium. (D) F3 consisted of an inoculum reactor (IR) containing cecal beads of chicken 3, connected to seven second-stage reactors (SSR) and continuously fed with 5% fermentation effluent of IR and 95% fresh fermentation mVL-3 medium. For activity, microbial composition and metabolites in reactor effluent samples were monitored daily using MiSeq and HPLC-RI, respectively, to show to the model stability and compared with the cecal inoculum.