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. 2021 Dec 20;12:780092. doi: 10.3389/fmicb.2021.780092

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Copyright © 2021 Asare, Greppi, Pennacchia, Brenig, Geirnaert, Schwab, Stephan and Lacroix.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Microbial composition and diversity in the inoculum reactor (IR) and four second-stage reactors (SSR) of F2 after initial stabilization and estimated from days 6–10 in period 1. (A,B) Microbial composition by 16S rRNA amplicon sequencing represented by relative abundance at phylum (A) and genus levels (B). When the genus assignment was not possible, the highest-level taxonomy assignment was shown. Values < 1% are summarized in the group “others”. (C,D) Principal coordinate analysis (PCoA) of reactor microbiota based on weighted (C) and unweighted (D) UniFrac analysis matrix. α-Diversity measured by (E) Shannon index and (F) observed ASVs.