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. 2021 Dec 20;12:780092. doi: 10.3389/fmicb.2021.780092

TABLE 1.

Conditions of initial bead batch colonization (A) and continuous fermentation (B) for different continuous cecal microbiota fermentations.

(A) Batch fermentation conditions for initial bead colonization
F1-A F1-B F2 F3
Nutritive medium* mVL-1 mVL-2 mVL-3 mVL-3
Supplementation SCFA mix SCFA mix SCFA mix SCFA mix
pH 6 6 6 6
Colonization—batch 1 20 h 20 h 20 h 20 h
Colonization—batch 2 6 h 6 h 6 h 6 h

(B) Continuous fermentation conditions

F1-A F1-B F2 F3

Nutritive medium* mVL-1 mVL-2 mVL-3 mVL-3
pH 6 6 6 6
Retention time 24 h 24 h 24 h 24 h
Total fermentation time (days) 13 13 70 82

*Composition presented in Supplementary Table 1. mVL-1, modified Viande Levure medium. mVL-2, modified Viande Levure medium with 2.5 g/L FOS. mVL-3, modified Viande Levure medium with 2.5 g/L FOS and 2.5 g/L citrus pectin.