TABLE 1.
(A) Batch fermentation conditions for initial bead colonization | ||||
F1-A | F1-B | F2 | F3 | |
Nutritive medium* | mVL-1 | mVL-2 | mVL-3 | mVL-3 |
Supplementation | SCFA mix | SCFA mix | SCFA mix | SCFA mix |
pH | 6 | 6 | 6 | 6 |
Colonization—batch 1 | 20 h | 20 h | 20 h | 20 h |
Colonization—batch 2 | 6 h | 6 h | 6 h | 6 h |
| ||||
(B) Continuous fermentation conditions | ||||
| ||||
F1-A | F1-B | F2 | F3 | |
| ||||
Nutritive medium* | mVL-1 | mVL-2 | mVL-3 | mVL-3 |
pH | 6 | 6 | 6 | 6 |
Retention time | 24 h | 24 h | 24 h | 24 h |
Total fermentation time (days) | 13 | 13 | 70 | 82 |
*Composition presented in Supplementary Table 1. mVL-1, modified Viande Levure medium. mVL-2, modified Viande Levure medium with 2.5 g/L FOS. mVL-3, modified Viande Levure medium with 2.5 g/L FOS and 2.5 g/L citrus pectin.