Skip to main content
. 2021 Dec 20;12:799444. doi: 10.3389/fphar.2021.799444

FIGURE 7.

FIGURE 7

Microscopic images identify the immunoreactivity of the antiapoptotic marker Bcl-2 in brainstem neuronal cells in the different studied groups (A) control group; (B) sham group (C) CCI group; (D) CCI + P. perfoliatus extract (Pot, 200 mg/kg, p.o) (E) CCI + P. perfoliatus extract (Pot, 400 mg/kg, p.o) (F) CCI + pregabalin. Arrowhead indicates dark brown staining of the cytoplasm of immuno-positive normal neurons and arrow indicates the negative immunostaining of the affected neurons. Scale bar, 50 μm × 400. (G) Bar chart presenting the quantification of the number of Bcl-2-positive normal neurons in all different studied groups. The immuno-positive neurons were counted from six animals/group in perceptive fields at ×400 magnification. One-way ANOVA was used for statistical analysis followed by a post-hoc Tukey test. Values are presented as means ± SEM.*p < 0.05 vs control group; # p < 0.05 vs CCI; @ p < 0.05 vs pregabalin.