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. 2021 Dec 20;8:756599. doi: 10.3389/fmolb.2021.756599

FIGURE 1.

FIGURE 1

TR1419-28BBζ CAR-T cells exhibited functional advances upon antigen stimulation. (A) Untransduced Mock T cells or TRI419-28ζ TRI419-BBζ CAR-T cells were evaluated by flow cytometry for the TRI419 scFv expression. (B) Representative CAR-T cell phenotyping was plotted based on CD45RA and CCR7 expression. CD45RA+CCR7+: naive-like T cells; CD45RA−CCR7+: central memory T cells; CD45RA−CCR7-: effector memory T cells; and CD45RA+CCR7-: effector T cells. (C) Qualification of inhibitory molecule PD-1 expression on CD4+ CAR and CD8+ CAR-T cells on day 7 post lentiviral transfection. (D) Flow cytometric analysis of CD137 expression on TR1419 CAR-T cells cultured overnight with plate-bound recombinant human TRAIL-R1 at various concentrations. (E) TR1419 CAR-T-cell proliferation, indicated by division of CFSE, after cultured with SW480 for 7 days was assessed by flow cytometry. The signal remaining value corresponded to the mean fluorescence intensity of the CFSE of mock T cells. (F–G) The expression of PD-1 on TRI419 CAR-T cells was analyzed by FACS after 7 days of coculture with tumor cells. Data are given in the mean ± SD of three independent experiments, *p < 0.05, **p < 0.01.