FIGURE 2.

TR1⁴¹⁹-28BBζ CAR-T cells induced significant cytolysis of TRAIL-R1–expressing tumor cells. (A) Cytotoxic activity of TR1⁴¹⁹-28BBζ CAR–transduced T cells or mock T cells against TRAIL-R1–positive tumor cell lines was determined by calcein AM release–based assay. The effector cells were cocultured for 6 h with target cells at E:T ratio of 10:1, 5:1, and 2.5:1, respectively. IFN-γ (B) and granzyme B (C) production by TR1⁴¹⁹-28BBζ CAR-T cells or mock T cells when cocultured with the indicated cells for 24 h was deducted by ELISA. Data are given in the mean ± SEM of three separate experiments. *p < 0.05, **p < 0.01, and ***p < 0.001.