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. 2021 Dec 20;8:756599. doi: 10.3389/fmolb.2021.756599

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Copyright © 2021 Nai, Du, Shen, Li, Huang, Han, Luo, Wang, Pang and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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scFv from TR1⁴¹⁹ CAR-mediated tumor cell apoptosis by binding with TRAIL-R1. (A) The expressions of TR1⁴¹⁹-28BBζ in Jurkat cells were detected by flow cytometry. (B) Cytotoxicity of TR1⁴¹⁹-28BBζ- and TR1⁴¹⁹Δζ-Jurkat cells against SW480 in the absence or in the presence of soluble TRAIL-R1 protein was determined by RTCA. (C) SW480 cocultured with TR1⁴¹⁹-28BBζ-and TR1⁴¹⁹Δζ-Jurkat cells for 2 h before subjected for FACS to identify apoptotic cell populations (Annexin V+/7-AAD− and Annexin V+/7-AAD+). (D) The expression of caspase-3 in SW480 was analyzed by flow cytometry, after incubation with effector cells for 2 h. Data are represented in mean ± SEM of triplicate wells, ***p < 0.001.