Figure 6.

MT1-expressing macrophages in tumor have increased metallothionein and pro-inflammatory gene expression and are associated with improved tumor event-free survival

(A) Violin plots show genes than achieved a p adj < 0.05 after statistical analyses with Wilcoxon Rank Sum Tests. Color of adjusted p value indicates the group where expression is higher (orange = tumor).

(B) Mean expression dot plot of metal ion transport genes in MAC-MT cluster separated by normal (N) or tumor (T) in rows. Size of circle indicates the percentage of cells expressing the genes and color indicates which group (normal or tumor) expresses higher (dark red) levels of the genes.

(C) Heatmap of mean AUCell enrichment of 27 macrophage-stimulation signatures split by normal or tumor. Row expression value is scaled from 0 to 1 and presented as a gradient from purple, blue, green to yellow.

(D) GSEA of KEGG pathways in tumor versus normal for LE-KLK4. Pathways were considered statistically significant if p value < 0.05 (marked by vertical dashed red line). Size of circles indicate normalized enrichment score (NES) and colors indicate if pathways achieved FDR < 0.25 starting from purple, blue, green to yellow as significance values decreases.

(E) String-DB analysis of leading edge genes from selected pathways enriched in tumor MAC-MT.

(F) Mean expression dot plot of CCL5, CXCL9, CXCL10 in MNP clusters and CXCR3 in lymphoid clusters. Size of circle indicates the percentage of cells expressing the genes and increasing expression (scaled from 0 to 1) corresponds to increasing color gradient from purple, blue, green to yellow.

(G) Representative immunofluorescence confocal microscopy of CD8 and HLA-DR in human prostate tumor section. Scale bars, 10 μm.

(H) Expression of CXCL9 and CXCL10 and prediction/label transfer scores of MAC-MT and CD8 cytotoxic cells in visium data of tumor prostate sections. (Bottom) Spatial correlation of CXCL9 and CXCL10 with MAC-MT or MAC-MT with CD8 cytotoxic cells in prostate cancer visium data. Only positive correlations are plotted; increasing value of correlation is shown as a gradient from white to red.

(I) Kaplan-Meier survival curve for TCGA-PRAD disease free index with deconvolved MAC-MT score. Samples were categorised into high (black, top 25%) and low (red, bottom 25%) of deconvolved score. Statistical analysis was performed with log rank test and p < 0.05 was considered statistically significant. See also Figures S8–S9 and Table S2.