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Characterization of ROP4 antigen. For western blot, a total of 10 and 2 μg of ROP4 VLP antigen were loaded into each lane. (A) Polyclonal mouse anti-T. gondii antibody was used to confirm the presence of T. gondii ROP4 protein (63 kD). (B) A transmission electron micrograph shows influenza virus-like morphology of ROP4 VLPs.
