Table 1.
Sequences and Features of Primers Used for RT-qPCR.
| Gene | Forward/Reverse | Sequence | Size(bp) | mRNA |
|---|---|---|---|---|
| E6 | 270 | GTATGGAACAACATTAGAACAGCAA | 81 | NC_001526.4 |
| 350 | AGTGGCTTTTGACAGTTAATACAC | |||
| E7 | 482 | GCATGGAGATACACCTACATTG | 273 | KX545363 |
| 754 | TGGTTTCTGAGAACAGATGG | |||
| Trx | 350 | CCACCTTTTGCGTCTTTCGG | 83 | XM_005758078.1 |
| 432 | CTCCACCAACGCCATAAGCG | |||
| P65 | 870 | GCAGGCTCCTGTGCGTGTCT | 286 | NM_021975.3 |
| 1155 | GGTGCTCAGGGATGACGTAAA | |||
| GLUT1 | 1071 | CTGGCATCAACGCTGTCTTC | 167 | NM_006516.3 |
| 1237 | GCCTATGAGGTGCAGGGTC | |||
| GAPDH | 50 | TTCTTTTGCGTCGCCAGCCGAG | 71 | XM_019023188.1 |
| 120 | CCAGGCGCCCAATACGACCAAA |
Abbreviations: mRNA, messenger RNA; qRT-PCR, quantitative reverse transcriptase-polymerase chain reaction.