Table 1.

Primer sequences, reaction conditions, and product size for RT-PCR

Gene	Sequence	Product size (bp)	Annealing (℃)	Cycles
GAPDH	F: ACC ACA GTC CAT GCC ATC AC R: TTC ACC ACC CTG TTG CTG TA	450	55	25
Collagen1	F: GAA AAC ATC CCA GCC AAG AA R: CAG GTT GCC AGT CTC CTC AT	270	57	23
Collagen3	F: CAG GTG AAC GTG GAG CTG C R: TGC CAC ACG TGT TTC CGT GG	661 849	57	23
Osteonectin	F: CCA GAA CCA CCA CTG CAA AC R: GGC AGG AAG AGT CGA AGG TC	161	57	23
Osteopontin	F: TCG CAG ACC TGA CAT CCA GT R: TCG GAA TGC TCA TTG CTC TC	267	57	32
BMP-2	F: GTC CAG CTG TAA GAG ACA CC R: GTA CTA GCG ACA CCC ACA AC	316	54	31

(RT-PCR: reverse transcription-polymerase chain reaction, GAPDH: glyceraldehyde 3-phosphate dehydrogenase, BMP-2: bone morphogenetic protein-2)