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. 2021 Jun 23;101(1):93–101. doi: 10.1177/00220345211017510

Figure 3.

Figure 3.

Gene set enrichment analysis of CRISPRa induced ST8SIA1 expression in HeLa cells. (A) The periodontitis-associated DNA elements at Region 1 (regulatory element tagged by rs3819872) and Region 2 (regulatory element tagged by rs2012722) regulate ST8SIA1 expression. Data show 3 biological replicates for each experiment (**P = 0.0017, ***P = 0.0002). CRISPR-activated cells with guide RNAs (gRNAs) that targeted the promoter and regulatory region tagged by rs2012722 were used for RNA-sequencing (RNA-seq). (B–D) Evidence plots (receiver operator characteristic curves) for the top 3 gene sets. Each panel corresponds to 1 gene set. The gray rug plot underneath each curve corresponds to genes sorted by P value, with the genes belonging to the corresponding gene sets highlighted in red (upregulated genes) or blue (downregulated genes). Bright red or bright blue indicates that the genes are significantly regulated. The area under the curve (AUC) corresponds to the effect size of the enrichment, with 0.5 being no enrichment and 1.0 being maximal possible enrichment.