Figure 2.

Chromogenic immunohistochemical images of paraffin sections of human bone marrow. (A) Human CD150-positive HSCs (brown) in their periarteriolar niche in healthy bone marrow adjacent to bone and (B) human CD123-positive LSCs (red, arrow head) in a HSC niche in bone marrow of a leukemia patient. Endothelial cells of capillaries are CD123 positive as well (asterisk). Hematoxylin staining in blue. a, lumen of arteriole. Bars, (A) 100 µm, (B) 25 µm. Tissue sections and staining are prepared according to Hira et al. ⁹ (A) and El Achi et al. ²⁴ Abbreviations: HSCs, hematopoietic stem cells; LSCs, leukemic stem cells.