Immunofluorescence images of human CD105-positive MSCs (green, arrows) in a periarteriolar HSC niche in healthy bone marrow adjacent to bone (A) and a periarteriolar GSC niche in a glioblastoma tumor (B). Monochrome images of CD105-positive MSCs in green and DAPI fluorescence (nuclei) in blue and the composition images in green and blue are shown. Smooth muscle cells in the tunica media (tm) of the arterioles are also CD105 positive. ta, Tunica adventitia of the arterioles. Tissue sections and immunofluorescence staining are prepared as described in Hira et al.
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Bars, 100 µm. Abbreviations: MSCs, mesenchymal stem cells; HSC, hematopoietic stem cell; GSC, glioblastoma stem cell; DAPI, 4′,6-diamidino-2-phenylindole.