Figure 2.

Representative immunostainings and OARSI grading of the cartilage at midterm (4 weeks) distraction treatment. (A) Representative images of Safranin-O/Fast Green staining and collagen type II immunohistochemistry from control (healthy), osteoarthritic (OA), and OA + KJD (Distraction) joints after 4 weeks of distraction of the tibial plateaus (tibia) and femoral condyles (femur). Scale bar = 100 µm. (S) = superficial layer; (I) = intermediate layer. (B) OARSI scoring of the histology after 4 weeks of distraction is given for all conditions, in cartilage from the femoral condyles and tibial plateaus. Asterisks indicate statistically significant differences between the indicated groups within the locations with at least medium effect sizes (*P < 0.05; **P < 0.01).