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. 2021 Sep 13;42(1):74–89. doi: 10.1177/0271678X211045438

Figure 3.

Figure 3.

Staining with collagen IV revealed increased number of string vessels in the cerebral cortex and corpus callosum of NemobeKO mice that remained elevated although to a much lesser extent in the recovery cohort (a). Retro-orbital injections of horseradish peroxidase and resultant visualization with DAB staining showed evidence of BBB leakage in NemobeKO mice that was no longer present after 3 months off tamoxifen (b). Infiltration of circulating leukocytes (CD45, red), not colocalized with microglial marker TMEM119 (green), was increased in all NemobeKO groups, particularly in the cortex of NemobeKO simvastatin (SV) group; this infiltration was decreased in the recovery cohort (c). Microbleeds were identified by magnetic resonance imaging as small black dots (white arrows) in NemobeKO mice regardless of treatment, while none were visualized in NemoFl mice, as confirmed ex vivo using an iron stain (d). N = 4–5 mice/group. One-way ANOVA with post-hoc Newman-Keuls multiple comparison tests were performed **p < 0.01, ***p < .001.