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. 2021 Aug 24;42(1):104–120. doi: 10.1177/0271678X211039617

Figure 7.

Figure 7.

The vector-encoded expression of the reporter gene eGFP in non-targeted cell types of extracerebral tissues is reduced by short regulatory sequences. (a) Representative images from lung, heart, liver, kidney and spleen 14 to 15 days after vector injection. AAV-transduced cells expressing eGFP under the control of the regulatory promoter sequences (green) were reduced compared to the results obtained with the control vector CAG-eGFP. CD31 was stained as a marker of endothelial cells (red). Scale bar, 100 µm. (b and c) The number of eGFP-positive cells in the lung (b) or heart (c) 14 to 15 days after vector injection are shown. Data are presented as means ± SD and were analyzed by one-way ANOVA (lung, F(4/11) = 6.645, p = 0.0057; heart, F(4/13) = 7.919, p = 0.0018), *p < 0.05, **p < 0.01 (Tukey’s multiple comparison test, n = 2–5 mice per vector). (d) Relative eGFP mRNA in liver 14 to 15 days after vector injection. Data are presented as means ± SD and were analyzed by one-way ANOVA (F(4/15) = 5.353, p = 0.007), *p < 0.0354 (Tukey’s multiple comparison test, n = 2-5 mice per vector).