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. 2021 Dec 14;23(2):49. doi: 10.3892/ol.2021.13167

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Copyright: © Komatsu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Cell proliferation assay and flow cytometric analysis. (A and B) shGIMAP2 and shMock cells were counted on seven consecutive days. The results are expressed as mean ± standard error of the mean of triplicate experiments. Cell growth was significantly inhibited (*P<0.05, Dunnett's test) in shGIMAP2 cells after 144 h of culture in HSC-2 cells and after 168 h of culture in HSC-3 cells. Cell growth was significantly (^†P<0.05, Student's t-test) higher in GIMAP2 transiently transfected cells (HSC-2 shGIMAP2-1 rescue and HSC-3 shGIMAP2-1 rescue) than in shGIMAP2 control cells (HSC-2 shGIMAP2-1 rescue control and HSC-3 shGIMAP2-1 rescue control) after 24 h of culture in HSC-2 cells and after 48 h of culture in HSC-3 cells. (C) Flow cytometric analysis showed that the percentage of shGIMAP2 cells in the G₁ phase was higher compared with shMock cells. sh, short hairpin; GIMAP2, GTPases of immunity-associated proteins 2.