Table 5.
Objective lenses and resolution
| Vendor | WSI device model | Objective type/numerical aperture (NA) | Image capture magnification (per objective lens) | Image resolution (μm/ pixel) (per image capture magnification) |
|---|---|---|---|---|
| 3D Histech | Pannoramic Desk II | 20x (NA 0.8) or 40x (NA 0.95) | 58x | 0.172 |
| Pannoramic MIDI II | 20x (NA 0.8) or 40x (NA 0.95) | 52x or 110x/31x or 62x | 0.172 or 0.087/0.325 or 0.162 | |
| Pannoramic Scan II | 20x (NA 0.8) or 40x (NA 0.95) | 52x or 110x/31x or 62x | 0.172 or 0.087/0.325 or 0.162 | |
| Pannoramic Confocal |
20x (NA 0.8) or 40x (NA 1.2) | 31x/62x | 0.325/0.162 | |
| Pannoramic 250 Flash III |
20x (NA 0.8) or 40x (NA 0.95) | 41x/82x | 0.242/0.121 | |
| Pannoramic 1000 | 20x (NA 0.8) or 40x (NA 0.95) | 41x/82x | 0.25/0.12 | |
| Grundium | Ocus | 20x (NA 0.4) | 0.48 | |
| Ocus 20 | 20x (NA 0.4) | 20x | 0.5 | |
| Ocus 40 | 40x (NA 0.75)a | 40x | 0.25 | |
| Huron | TissueScope LE120 |
20x (NA 0.75) | 20x/40x | 0.4/0.2 |
| TissueScope iQ | 20x (NA 0.75) | 20x/40x | 0.4/0.2 | |
| TissueScope CF | 20x (NA 0.6) | 1x/2.5x/5x/10x/20x/40x | 10.0/5.0/2.0/1.0/.50/.25 | |
| Tissuescope LE | 20x (NA 0.75) | 20x/40x | 0.4/0.2 | |
| TissueScope PE | 20x (NA 0.75) | 1x/2.5x/5x /10x/20x/40x | 10.0/5.0/2.0/1.0/.50/.25 | |
| Hamamatsu | NanoZoomer SQ | 20x (NA 0.75) | 20x/40x | 0.46/0.23 |
| NanoZoomer S60 | 20x (NA 0.75) | 20x/40x | 0.46/0.23 | |
| NanoZoomer S210 | 20x (NA 0.75) | 20x/40x | 0.46/0.23 | |
| NanoZoomer XR | 20x (NA 0.75) | 20x/40x | 0.46/0.23 | |
| NanoZoomer S360 | 20x (NA 0.75) | 20x/40x | 0.46/0.23 | |
| Mikroscan | MikroScan SL5 | 2x (NA 0.06), 4x (NA 0.10), 10x (NA 0.25), 20x (NA 0.50), and 40x (NA 0.65) | 2x/4x/10x/20x/40x | 4.54/2.27/0.908/0.454/0.227 |
| Motic | EasyScan Pro 6 | 10x (NA 0.3), 20x (0.75), 40x (0.75) | 20x/40x/80x | 0.52/0.26/0.13 |
| EasyScan One | 10x (NA 0.3), 20x (NA 0.75), 40x (NA 0.75) | 20x/40x/80x | 0.52/0.26/0.13 | |
| EasyScan Infinity 100 | 10x (NA 0.3), 20X (0.75), 40x (0.75) | 20x/40x/80x | 0.52/0.26/0.13 | |
| Objective Imaging |
Glissando 20SL (medium capacity) 0.75) | Single lens 20x (NA 0.75), 40x (NA | 20x/40x | 0.55/0.275 |
| Glissando POL | Single-plan fluorite objective lens for polarized light applications; choose from 4x, 10x, 20x, or 40x magnification; for bright-field: 20x lens/ NA 0.75 Plan Apo | 4x/10x/20x/40x | 1.23/0.49/0.25/0.12 | |
| Glissando Desktop Scanner |
Single lens, 20x/0.75 NA Plan Apo, or 40x/0.75 NA Plan Fluor | 20x/40x | 0.55/0.275 | |
| Leica | Aperio VERSA | HCb Plan Fluotar: 1.25x, 5x, 10x, 20x, 40x, 63x (oil), 63x (dry) HC Plan Apo: 20x, 40x (dry), 40x (oil) |
||
| Aperio AT Turbo | 20x/0.75NA Plan Apo (40x scanning with 2x automatic optical mag changer) | 20x/40x | 0.5/0.25 | |
| Aperio AT2 | 20x/0.75 NA Plan Apo (40x scanning with 2x optical mag changer) | 20x/40x | 0.5c/0.25 | |
| Aperio CS2 | 20x/0.75 NA Plan Apo (40x scanning with 2x optical magnification changer) | 20x/40x | 0.5/0.25 | |
| Aperio GT 450 | 40x | Overview image/40x | 13.0/0.26 | |
| Aperio LV1 | 1.25x (NA 0.03), 5x (NA 0.16) and 20x (NA 0.4) ECd Plan-Neofluar | Overview image/2.5x/5x 10x/20x/40x/63x (digital magnification) | 10.0/2.16/1.08/0.54/0.27 0.138/0.086 | |
| Philips | UFS | 20x NA of 0.75 Plan Apo | 40x | 0.25 |
| Roche | Ventana DP200 | 20x and 40x | 40x | 0.23 |
| Ventana iScan | 4x (0.1NA), 10x (0.3NA), 20x | 4x/10x/20x/40x | 2.5/1.0/0.46/0.23 | |
| Coreo | (0.50NA), 40x (0.75NA) | |||
| Ventana iScan HT | 20x and 40x | 40x | 0.23 | |
| Sakura | VisionTek (Plan NeoFluar) | 2.5x(0.075 NA), 10x (0.3 NA), 20x (0.5 NA), 40x (magnification not objective) | Overview camera resolution at 0.45x/Live view and scanning camera resolution at 2.5x/10x/20x | 12.0/2.20/0.550/0.275 |
| VisionTek M6 | 2.5x, 5x (0.16 NA), 10x, 20x (0.5 NA), 40x (0.75 NA), 63x (magnification not objective) | Overview camera:e 0.45x = Live view and scanning camera:f 2.5x/5x/10x/20x/40x | Overview camera: 12.0 Live view and scanning camera: 2.2/1.10/0.55/0.275/0.138 |
|
| OptraScan | Ultra 320 | 40x (0.75 NA) | 20x/40x | 0.50/0.25 |
aAchieves 40x magnification via utilization of a 0.75 NA 20x wide field objective
bLeica Microsystems HC System (Harmonic Compound System)
c0.5 μm per pixel is equivalent to 50,000 pixels per inch by using a 20x objective for the Leica Aperio AT Turbo, Aperio AT2, Aperio CS2 WSI Devices
d“Enhanced Contrast” Plan-Neofluar objective from Zeiss
eOverview Camera (Sakura VisionTek): CMOS; 5MP (2560x1920); pixel size 2.2 μm x 2.2 μm
fLive view and scanning camera (Sakura VisionTek): CCD camera; pixel size 5.5 μm x 5.5 μm