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. 2021 Dec 20;8:756629. doi: 10.3389/fvets.2021.756629

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Copyright © 2021 Feng, Wang, Zhang, Zhang, Zou, Zhang, Wang, Guo, Qiu, Wang, Xin and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of doxycycline or SGDL granule and both on IgA concentration in tracheal washing using ELISA kit (A) and AOD using ImageJ analysis of the expression of IgA⁺ cells in the tracheal mucosa of chicken (B). Group I served as a control. Broilers in Group II were given doxycycline whereas Group III was given SGDL granule through drinking water. Broilers in Group IV were given SGDL granule and doxycycline in drinking water. Extremely significant (p < 0.01) different values are shown with **. Significantly (p < 0.05) different values are shown with *, and no signal was not significantly (p > 0.05) different between the groups at those time points. SGDL, Shegandilong.