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. 2021 Dec 20;8:756629. doi: 10.3389/fvets.2021.756629

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Copyright © 2021 Feng, Wang, Zhang, Zhang, Zou, Zhang, Wang, Guo, Qiu, Wang, Xin and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative mRNA expressions of IL-1β, IL-6, TNF-α, and IFN-γ in tracheas at 1, 3, 5, and 7 dpi. (A–D) Represent IL-1β, IL-6, TNF-α, and IFN-γ mRNA relative expression in trachea, respectively. Group I served as a control. Broilers in Group II were given doxycycline whereas Group III was given SGDL granule through drinking water. Broilers in Group IV were given SGDL granule and doxycycline in drinking water. It was intraocularly and intranasally challenged by IBV at day 28. Extremely significant (p < 0.005) different values are shown with ***, extremely significant (p < 0.01) different values are shown with **, significantly (p < 0.05) different values are shown with *, and no signal was not significantly (p > 0.05) different between the groups at those time points. SGDL, Shegandilong.