Figure 1.

Study design. We exposed adult male and female mice from six strains in sex- and age-matched pairs to filtered air (FA) or 2 ppm ozone (O₃) for 3 h and euthanized 21 h later. We performed bronchoalveolar lavage (BAL) to collect cells and protein from the airspace for quantitative inflammatory and injury phenotyping. We also collected airway macrophages by adherence from BAL cells from each mouse and performed gene expression by RNA-seq and, in CC003 and CC017, profiled chromatin accessibility using assay for transposase-accessible chromatin using sequencing (ATAC-seq). (n = 3 mice per sex/treatment/strain except CC0039 where n = 4 females exposed to O₃ and 1 male exposed to FA and CC003 where n = 1 female and 2 males exposed to FA).