Figure 3.

Respiratory cytokine responses are altered by O₃ exposure and vary by strain. Molecular markers of inflammation eotaxin-1 (CCL11; A), IL-6 (B), IL-10 (C), IP-10 (CXCL10; D), KC (CXCL1; E), LIX (CXCL5; F), and MIP-1β (G) were measured using a multiplex cytokine detection kit. Data are presented as box-and-whiskers plots, which display the distribution from the minimum, first quartile, median, third quartile, and maximum. Individual data points are overlaid, with circles representing FA-exposed mice and triangles representing O₃-exposed mice. Closed points are female mice, whereas open points are male mice. Points below the assay limit of detection were excluded from analysis. Raw data are available in Supplemental Table S1. Eotaxin-1, KC, and IL-6 also displayed significant strain-by-O₃ exposure interactions assessed by a likelihood-ratio test (A: P < 1 × 10⁻⁷, B: P < 0.005, C: P < 0.0005). (n = 2 mice per sex/strain/treatment for all strains except CC003 where n = 3 females/1 male exposed to O₃ and n = 1 female/2 males exposed to FA and CC0039 where n = 3 females/1 male per treatment; *P < 0.05, **P < 0.005, ***P < 0.0005 for within strain contrasts (t tests) between FA vs. O₃). FA, filtered air; O₃, ozone.