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Concurrent analysis of apoptosis and cell cycle progression. Exponentially growing cultures of c-myc+/+ (A, B, and C) and c-myc−/− (D, E, and F) cells were treated with etoposide (Eto) or cisplatin (Cis) as indicated, harvested at the indicated time points, processed for TUNEL using the Apo-Direct kit (Pharmingen), counterstained with propidium iodide, and analyzed by two-parameter flow cytometry.
