Fig. 3.

Detection of entry inhibitors and antibodies against SARS-CoV-2 pseudovirus infection. A) E-64d blocked SARS-CoV-2 S variants pseudovirus entry. 293T-ACE2 cells were pre-incubated with E-64d (about 3-fold serially diluted) and subsequently inoculated with pseudovirions. RLU were detected after 72 h post-pseudovirus inoculation. B) Camostat mesylate slightly blocked SARS-CoV-2 S variants pseudovirus entry. 293T-ACE2 cells were pre-incubated with camostat mesylate (about 3-fold serially diluted) and subsequently inoculated with pseudovirions. RLU were detected after 72 h post-pseudovirus inoculation. Antibodies targeted RBD neutralized the SARS-CoV-2 S variants pseudovirus. The S WT C), D614G D), N501Y E) and Delta F) pseudovirus were pre-incubated with mAb-A or mAb-B (about 3-fold serially diluted), and subsequently inoculated with 293T-ACE2 cells. RLU were detected after 72 h post-pseudovirus inoculation. Data in A) B) C) D) E) and F) are shown as mean ± SE of three independent experiments.