Figure 2.

Humoral responses elicited by SARS-CoV-2 SAM (LNP) vaccine in mice

Pseudovirus-based spike 50% neutralization titers (pVNT₅₀) of immunized mice sera or human COVID-19 convalescent sera (HCS) against Wuhan (A), Alpha (B.1.1.7) (C), Beta (B.1.351) (D), or Delta (B.1.617.2) (E) spike sequences. A panel of 22 HCS was tested against Wuhan, Alpha, and Beta, whereas a panel of 21 HCS (same panel minus one subject) was tested against Delta. Mouse sera were collected 3 weeks after the first vaccination (3wp1) and 2 weeks after the second vaccination (2wp2). (B) SARS-CoV-2 50% virus neutralization titers (VNT₅₀) measured with a mNeonGreen reporter virus-based assay in sera collected 2wp2 from immunized mice or in a panel of COVID-19 HCS (n = 22). The dotted line indicates the limit of detection. Geometric means of each group ±95% confidence interval are shown. Each dot represents individual samples. Bars under p value asterisks indicate significantly different groups comparisons with ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001.