Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 3;23:6. doi: 10.1186/s13059-021-02586-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — Guide mismatches at position 10 and 11 abolish GIGS, indicating AGO dependence. a Illustration of multi-guide expression from TRV targeting the PDS transcript. For each of the 28-nt guides (crRNA1, crRNA2, crRNA 3) a variant m-guide 1 was designed. For mg 1[mm5,6], each crRNA contained two base pair mismatches at positions 5,6, for mg 1[mm10,11] mismatches at positions 10,11, and mg 1[mm21,22] contained mismatches at positions 21,22. b Representative images of leaves following TRV systemic delivery of m-guide 1 targeting PDS, in addition to the three variants of m-guide 1. TRV expressing a non-targeting guide (NT-guide) and TRV with a region of antisense sequence to PDS (asPDS) served as controls. c SPAD meter readings from photobleached (loss of green color) leaf samples. Data collected from a total of six independent leaves from two experiments. d Quantification of the PDS transcript using qPCR for the same samples as measured in c. Data standardized to an endogenous transcript and normalized to TRV expressing NT-guide. Statistical comparisons were made between the NT-guide and each treatment using a one-sided Mann-Whitney U test with Benjamini-Hochberg (BH) multiple testing correction. Samples with p values less than 0.05 (*) and 0.01 (**) are indicated. n.s., non-significant difference (p > 0.05)