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. 2022 Jan 3;23:6. doi: 10.1186/s13059-021-02586-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — The Cas13 DR sequence is not required to elicit GIGS. a Schematic representation of modified PDS m-guide1 sequences where the LbuCas13a DR was replaced with Arabidopsis miR170 stem-loop (stem-loop, 24 nt), the miR170 without the four base pair stem (loop, 16 nt), a random sequence (random, 20 nt), and without any intervening sequence (contiguous). b Representative images of N. benthamiana leaves after 2 weeks of TRV systemic delivery. The guide delivered for each TRV construct is labeled above or below the image. The bottom row shows leaves that all expressed a version of PDS m-guide 1. c Chlorophyll content (leaf greenness) measured with SPAD meter 2 weeks after systemic delivery of crRNA. d Relative PDS transcript levels quantified using qPCR, compared between the infiltration control (TRV_empty) and each treatment. Statistical comparisons were made using one-way ANOVA with multiple comparisons of treatments by means of Tukey performed using honestly significant difference. Treatments with the same letter are not significantly different