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. 2022 Jan 3;21:6. doi: 10.1186/s12943-021-01473-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Single-cell enrichment of stemness/EMT gene signatures in sorafenib resistant cells. A IC50 of Huh7 parental (Huh7-P) and sorafenib-resistant (Huh7-R) cells. B-C Heatmaps showing the expression profiles of stemness/EMT (B) and Notch signalling related genes (C) as determined by single cell-RNA seq analysis. D Scatter plot showing the single-cell expression levels of Notch1–3 receptors in Huh7-P and Huh7-R cells. E t-SNE plots along with single-cell expression levels indicated on a purple/grey colour scale of cell-cycle related genes. The black circle represents the Huh7-R cell population with low gene expression. F tSNE plot representing the distribution of Huh7-P (red) and Huh7-R (green) cells. Arrows indicate a few Huh7-P cells (candidate stem cells) that are clustered together with Huh7-R cells. G Dot-plot showing the expression levels of stemness/EMT genes in Huh7-P candidate stem cells and Huh7-R cells