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. 2022 Jan 3;21:6. doi: 10.1186/s12943-021-01473-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Role of ZFAS1 in the induction of stemness and sorafenib resistance. A Bulk mRNA expression levels of stemness and notch signaling related genes in Huh7 cells with or without ZFAS1 transfection presented as a heatmap. B Representative images of tumour colony of Huh7 cells with or without siRNA transfection. C-D Quantitative analysis of tumour colonies (C) and sphere numbers (D). Data represented as mean ± standard deviation (SEM). **: p < 0.01 (compared to Ctrl). E Representative images of cell growth of Huh7 cells with or without siRNA transfection. Scale bar = 100 μm. F Viability of Huh7 cells with or without siRNA transfection following sorafenib (5 μM and 10 μM) treatment. Data represented as mean ± SEM. *: p < 0.05; **: p < 0.01 (compared to Ctrl). G Representative images of apoptotic percentage of Huh7 cells with or without siRNA knockdown after sorafenib treatment. H Quantitative analysis of G. Data represented as mean ± SEM. *: p < 0.05 (compared to Ctrl)