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. 2001 Aug;21(15):4938–4948. doi: 10.1128/MCB.21.15.4938-4948.2001

FIG. 2.

FIG. 2

Replication fork movement pauses at the HML ARS cluster in chromosome III. (A) Schematic illustration of replication fork pause signals (spots 1, 2, and 3) detected on the Y arc after 2D gel electrophoresis of a DNA fragment containing the HML ARS cluster. A 3.9-kb EcoRI/FspI genomic fragment containing the centrally located HML ARS cluster (ARS302-ARS303-ARS320 [302 303/ 320]) is diagrammed. ARS elements are indicated as small arrows. The filled box indicates part of the HML cassette, and the shaded arrow indicates part of the CHA1 gene. The direction of fork migration is indicated. Major (1) and minor (2 and 3) pause sites are depicted as accumulated replication forks. (B to D) Two-dimensional gel electrophoresis of replication intermediates from particular strains probed for specific restriction fragments shown in the maps. Arrowheads in the 2D gel patterns denote the center of the pause signal. (B) A 3.9-kb EcoRI/FspI genomic fragment from the parental strain YPH98; (C) a 5.4-kb EcoRI genomic fragment from the YPH98 strain; (D) a 2.8-kb EcoRI/FspI genomic fragment from a mutant strain with a 1.07-kb deletion of part of the HML cassette (YWY6). (E) A 3.35-kb EcoRI genomic fragment from the YWY6 strain.