FIG. 3.

Mapping of a replication fork pause site associated with ARS301 on the left end of chromosome III. Arrowheads denote the center of the pause signal. (A) A 5.4-kb XhoI genomic fragment from the YPH98 strain. ARS301 is located off center to the left of the XhoI fragment. (B) A 3.05-kb BamHI/FspI genomic fragment from the YPH98 strain. ARS301 is located off center to the right of the BamHI/FspI fragment. In both maps, a fork that moves unidirectionally from the centromeric side (right) is responsible for the replication of ARS301 region.