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The exosomal gp96 triggered cell pyroptosis in CD8+ T cells. (A) Real-Time qPCR was performed to determine the generation of IL-1β and IL-18 in the CD8+ T cells. (B) The secretion of IL-1β and IL-18 in the CD8+ T cells’ supernatants were measured by ELISA. (C) The expression levels and cellular localization of Gasdermin D in the CD8+ T cells were determined by using the immunofluorescence staining assay. *P < 0.05.
