Table 6.

Literature review on DoE-based optimization of E. coli culture condition

Host Organism	Product	Design	Studied factors	Product increase	Reference
E. coli SHuffle T7	Biomass (SU-INS)	PBD, CCD	C, N sources, inorganic salts, pH	2.3X (shake flask), 5.9X (fermentor)	Present study
E. coli BL21 (DE3)	Phytase	CCD	C, N sources, salt	1.2X	[18]
E. coli (ATCC no. 11303)	L-Asparaginase	OFAT, CCD	C, N sources, ions, inoculum age and size	10X	[19]
E. coli BL21 (DE3)	L-Asparaginase II	FFD, CCFD	C, N sources, ions	NA	[20]
E. coli BL21 (DE3)	Streptokinase	PBD, CCD	C, N sources, ions	7X	[21]
E. coli	Dihydrolipohyl Dehydrogenase	CCFD	C, N sources, inorganic salts, pH	1.2X (shake flask), 1.7X (fermentor)	[22]
E. coli DH5α	Rheumatoid arthritis DNA vaccine	OFAT, PBD, BBD	C, N sources, salts, trace elements, pH, inoculum size, temperature	51.9%	[23]
E. coli MET-3	L-Methionine	PBD, BBD	C, N sources, salts	1.5X (shake flask), 6.4X (fermentor)	[24]
E. coli BL21 (DE3)	Reteplase	BBD	Temperature, Agitation, pH	2X	[25]
E. coli Δwaaf	Colanic acid	FFD, CCD	C, N sources, inorganic salts	12X	[26]
E. coli BL21 (DE3)	γ-cyclodextrin glycosyltransferase	OFAT, PBD, Steepest ascent path, BBD	C, N sources, inorganic salts, pH	2.83X	[27]