FIG. 1.

Cells overexpressing CDC5 or cdc5N209A are multinucleate. To induce Cdc5 (A) or cdc5-N209A (B), 2% galactose was added to mid-logarithmic-phase cultures of strains JC256 (GAL-CDC5) and C1039 (GAL-cdc5N209A GFP-TUB1) for the indicated times. To induce GFP-Tub1 expression, the same cells were then resuspended in SC-methionine plus galactose for 2 h. The cells were fixed and stained with DAPI to visualize nuclei. It should be noted that the frequency of multinucleate cells as visualized here does not reflect the true distribution in these cultures. Rather, these particular fields were chosen in order to demonstrate the variety of multinucleate cells in the culture. Bars, 10 μm.