TABLE 1.
Strains used
| Strain | Genotype (source) |
|---|---|
| W303-1A | MATaade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 (R. Rothstein) |
| W303-1B | MATα ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 (R. Rothstein) |
| AA1120 | W303-1A cdh1/hct1::HIS3 PDS1-HA-LEU2::pds1 (A. Amon) |
| IAY18 | MATaspc42Δ1::LEU2 TRP1::SPC42-GFP (J. Kilmartin) |
| JC1195 | MATα leu2 ura3 his3-Δ200 (J. Cooper) |
| JC256 | W303-1A ura3::(GAL-CDC5-HA-URA3)3 (J. Charles) |
| JC278 | W303-1A ura3::(GAL-cdc5-N209A-HA-URA3)3(J. Charles) |
| K3371 | W303-A clb2::LEU2 trp1::GAL-CLB2-TRP1 (Kim Naysmith) |
| K6451 | W303-1A cdc5-3(msd2-1::URA3) omns PDS1::LEU2 (K. Nasmyth) |
| MAY1 | W303-1A hsl1Δ1::URA3 (M. Grunstein) |
| MAY2 | W303-1A hsl1Δ1::URA3 swe1::LEU2 (M. Grunstein) |
| MAY3 | W303-1A hsl7Δ1::URA3 (M. Grunstein) |
| MAY4 | W303-1A hsl7Δ1::URA3 swe1::LEU2 (M. Grunstein) |
| NEY100X | MATα hsl1::LEU2 ura3 leu2 |
| CH179 | MATα ura3::GFP-TUB1-URA3 his3 leu2 |
| CH199 | W303-1A bar1::URA3 CDC5-ProA-HIS3-URA3 |
| CH452 | MATα SWE1-GFP-HIS3 leu2 ura3 his3-Δ200 (GFP-tagged SWE1 in JC1195) |
| CH459 | MATabar1 ura3::(GAL-cdc5-N209A-HA-URA3)3 SWE1-ProA-URA3-HIS3 (ProA tag of SWE1 in JC278) |
| CH473 | MATaura3::(GAL-CDC5-HA-URA3)3 SWE1-ProA-URA3-HIS3 (ProA tag of SWE1 in JC256) |
| CH474 | W303-1A swe1::GAL-SWE1-HA-LEU2 (GAL-SWE1-HA integrated at the SWE1 locus in W303-1A using pSWE1-41, from D. Lew) |
| CH499 | W303-1A ura3::(GAL-cdc5-N209A-HA-URA3)3 swe1::(GAL-SWE1-HA-LEU2) (GAL-SWE1-HA integrated at the SWE1 locus in YJC278 using pSWE1-41, from R. Booher) |
| CH507 | MATα ura3::(GAL-CDC5-HA-URA3)3 hsl1::LEU2 (segregant of JC256 × NEY100X) |
| CH508 | MATaura3::(GAL-cdc5-N209A-HA-URA3)3 hsl1::LEU2 ADE2 his3 (segregant of NEY 100X × JC278) |
| CH572 | MATaura3::(GAL-cdc5-N209A-HA-URA3)3 SWE1-GFP-HIS3 his3 leu2 ura3 (segregant of JC278 × CH452) |
| CH573 | MATaura3::(GAL-CDC5-HA-URA3)3 SWE1-GFP-HIS3 his3 leu2 ura3 (segregant of JC256 × CH452) |
| CH618 | MATaura3::(GAL-cdc5-N209A-HA-URA3)3 hsl7Δ1::URA3 his3 leu2 ura3 trp1-1 (segregant of CH696 × MAY3) |
| CH667 | W303-1B cdc5-3(msd2-1::URA3) swe1::LEU2 (SWE1 disrupted with pSWE1-10g, from R. Booher) |
| CH696 | W303-1B ura3::(GAL-cdc5-N209A-HA-URA3)3 (segregant of JC278 × W303-1B) |
| CH745 | W303-1B cdc5-3(msd2-1::URA3) (segregant of KN6451 × W303-1α) |
| CH798 | W303-1A swe1::LEU2 (segregant of MAY4 × W303-1B) |
| CH810 | W303-1B cdh1(hct1)::HIS3 (segregant of AM1120 × W303-1B) |
| CH895 | W303-1A cdh1(hct1)::HIS3 swe1::LEU2 (segregant of CH798 × CH810) |
| CH896 | W303-1B cdh1(hct1)::HIS3 swe1::LEU2 (segregant of CH798 × CH810) |
| CH943 | MATα swe1::LEU2 leu2 ura3 his3-Δ200 (segregant of JC1195 × CH798) |
| CH964 | W303-1A cdh1(hct1)::HIS3 swe1::LEU2 pRS424 (CH895 transformed with pRS424) |
| CH965 | W303-1A cdh1/hct1::HIS3 swe1::LEU2 pRS424-SIC1 (CH895 transformed with pRS424-SIC1) |
| CH968 | W303-1A cdh1/hct1::HIS3 swe1:LEU2 ura3::GFP-TUB1-URA3 (GFP-tagged TUB1 using pAFS92, from A. Straight, in CH895) |
| CH1039 | MATa/α ura3::GFP-TUB1-URA3/ura3::(GAL-cdc5-N209A-HA-URA3)3 (JC278 × CH179) |
| CH1173 | W303-1A cdh1/hct1::HIS3 swe1::LEU2 clb2::LEU2 (segregant of CH896 × K3371) |
| CH1175 | MATaswe1::LEU2 GAL-CLB2::TRP1 (segregant of CH943 × K3371) |
| CH1201 | MATaspc42Δ1::LEU2 TRP1::SPC42-GFP ura3::(GAL-cdc5-N209A-HA-URA3)3(segregant of CH696 × IAY18) |
| CH1221 | W303-1A ura3::(GAL-cdc5-N209A-HA-URA3)3 cdh1/hct1::HIS3 (segregant of JC278 × CH810) |
| CH1224 | W303-1A ura3::3(GAL-CDC5-HA-URA3)3 cdh1/hct1::HIS3 (segregant of JC256 × CH810) |