TABLE 2.
Multinucleate formation in CDC5 and cdc5-N209A overexpression strains
| Strain | Distribution (%)a
|
|
|---|---|---|
| 1 nucleus/mother | 2 nuclei/mother | |
| WT | 99.94 | 0.06 |
| WT + Gal (4) | 99.94 | 0.06 |
| GAL-CDC5 + Gal (0) | 99.7 | 0.3 |
| GAL-CDC5 + Gal (4) | 97.5 | 2.5 |
| GAL-cdc5-N209A + Gal (0) | 99.9 | 0.1 |
| GAL-cdc5-N209A + Gal (4) | 99.1 | 0.9 |
| cdh1/hct1 + Gal (0) | 98.2 | 1.8 |
| cdh1/hct1 + Gal (4) | 98.8 | 1.2 |
| GAL-CDC5 cdh1/hct1 + Gal (0) | 98.9 | 1.1 |
| GAL-CDC5 cdh1/hct1 + Gal (4) | 78.6 | 21.4 |
| GAL-cdc5-N209A cdh1/hct1 + Gal (0) | 99.0 | 1.0 |
| GAL-cdc5-N209A cdh1/hct1 + Gal (4) | 85.0 | 15.0 |
a
The number of nuclei per mother cell in haploid cells was determined by DAPI staining of asynchronous cultures at 30°C in mid-log phase. Greater than 1,000 cells were counted for each strain. All strains used were derivatives of W303 (wild type [WT]). All cells were grown in rich medium containing raffinose; where indicated, galactose was added for the time periods (hours) shown in parentheses.