Fig. 7.

Silencing TINCR impairs HCC tumorigenesis and resistance to oxaliplatin in vivo. A, TINCR expression in HepG2 and HuH7 cells transfected with stable sh-TINCRs or scrambled control. B-D, HuH7 cells with or without stable TINCR knockdown were transplanted into the axilla of nude mice. Once the tumor nodes grew to 100 mm³, the mice were randomly divided into four groups (n = 6/group) and intraperitoneally injected with 5% glucose solution (GS) or oxaliplatin (OXA) (3 mg/kg) every 3 days. Representative images (B), tumor volume growth curves (C), and weight of the xenograft tumors(D). E-F, Representative images (E) and quantification (F) of immunohistochemistry analysis of ST6GAL1 expression level in tumors from HuH7 cells stably transfected with sh-TINCR 2# and scramble control. Data are expressed as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; NS: no significance