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. Author manuscript; available in PMC: 2022 Jan 3.
Published in final edited form as: Cancer Res. 2019 Dec 10;80(4):857–867. doi: 10.1158/0008-5472.CAN-19-1991

Figure 2. Missense alleles in BRIP1 increase sensitivity to ICL damage.

Figure 2.

A) Representative karyotypes of independent HeLa clones after exposure to MMC [60nM]. Chromosomal breaks, gaps, radials (red arrows) and acentric fragments (yellow arrows) were observed in −/−, A349P/-, P47A/- and D791V/−. The average number of breaks/gaps, radial formations and acentric fragments per metaphase spread were calculated for each genotype (n=15 spreads per cell line). Statistical significance determined by two way ANOVA with a Bonferroni posttest to BRIP1+/+ (*** p<0.0001). B) Overlay of representative cell cycles from independent HeLa clones with and without MMC treatment. Statistical significance calculated by averaging percentage of cells in G2/M from 3 replicates (one-way ANOVA with Tukey’s multiple comparison test).