Fig. 1.

Capturing the process of alveolarization at a single-cell resolution. (A) Schematic of time points sampled, overlaid onto histologically identified stages of murine lung development. (B) Experimental workflow: at least four mice were pooled per time point; lungs were harvested and single-cell suspensions were generated by enzymatic digest; viable, CD45− Ter119− cells were sorted by FACS for scRNA-seq library preparation. (C) UMAP embedding of all 102,571 cells that clustered, annotated by cell type. (D) Hallmark identifier genes for each cluster are plotted where the size of the dot indicates the proportion of cells within a cluster expressing that gene, and level of blue saturation indicates relative expression level.