Skip to main content
. Author manuscript; available in PMC: 2022 Jan 3.
Published in final edited form as: Sci Signal. 2021 Apr 20;14(679):eabc0425. doi: 10.1126/scisignal.abc0425

Figure 1. S1P promotes CLIC1 and CLIC4 localization to plasma membrane in endothelial cells.

Figure 1.

(A) RNA sequencing of human umbilical vein endothelial cells (HUVEC) revealed that only CLIC1 and CLIC4 are expressed. (B and C) HUVECs were infected with HA-CLIC4 (B) and HA-CLIC1 (C), and immunofluorescent studies using HA-antibody were performed after 2min and 5min of 1μM S1P stimulation. Arrows indicate accumulation of HA-CLIC4 and HA-CLIC1 at the plasma membrane. Images are confocal microscopy analysis and representative of three independent experiments. Scale bar = 30 μm