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. Author manuscript; available in PMC: 2022 Jan 3.
Published in final edited form as: Sci Signal. 2021 Apr 20;14(679):eabc0425. doi: 10.1126/scisignal.abc0425

Figure 4. CLIC1 and CLIC4 function uniquely in regulating Rac1 activity.

Figure 4.

(A and B) HUVECs were serum starved for 2hrs then stimulated with 1μM S1P. Trans-endothelial electrical resistance (TEER) was monitored for 2hrs post treatment. Resistance was normalized with respect to time of S1P addition. Graphs are representative of three independent experiments. (C and D) Boyden chamber migration assay towards 1μM S1P for 6hrs after 3hrs of serum starvation. Quantifications of the number of migrated cells per field are shown. All data (means ± SEM) were generated from at least three biological replicates, and significance was determined by ANOVA with Bonferroni correction for multiple comparisons test (** p<0.01, ns: not significant). (E and F) HUVECs were serum starved for 3hrs then treated with 1μM S1P for 5min. Rac1 activity was measured by G-LISA assay and normalized to unstimulated cells for each condition (ANOVA with Bonferroni correction for multiple comparisons test, n=3, ***p<0.001).