Figure 6. ACE2 decoys carrying v2.4 mutations bind S proteins from multiple highly transmissible SARS-CoV-2 VOCs.

(A) Human Expi293F cells expressing myc-tagged S from four SARS-CoV-2 variants (Wuhan, B.1.1.7/alpha, P.1/gamma, and B1.351/beta) were incubated with monomeric sACE2–8h (black) or dimeric sACE2₂-IgG1 (grey) and bound protein was detected by flow cytometry. N=2. (B) Binding of mAbs versus sACE2₂.v2.4-IgG1 to S proteins of four VOCs (B.1.1.7/alpha, P.1/gamma, B1.351/beta, and B.1.617.2/delta) measured by flow cytometry. N=2.