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[Preprint]. 2021 Dec 29:rs.3.rs-1211792. [Version 1] doi: 10.21203/rs.3.rs-1211792/v1

Figure 1. B.1.1.529 is attenuated in different mouse models of SARS-CoV-2 infection.

Figure 1

a, Far left: Weight change in 129 mice inoculated via intranasal route with 104 (purple circles, n = 6) or 105 (blue circles, n = 6) FFU of B.1.1.529 (strain hCoV-19/USA/WI-WSLH-221686/2021) or 105 FFU of WA1/2020 N501Y/D614G (black circles, n = 6). Center left: Weight change in mock-infected 129 mice (grey circles, n = 4) or 129 mice inoculated intranasally with 103 (red circles, n = 5) PFU of B.1.1.529 (strain hCoV-19/USA/NY-MSHSPSP-PV44476/2021) or B.1.351 variant of SARS-CoV-2 (black diamonds, n = 3). Center right: Weight change in 129 mice inoculated intranasally with 106 PFU of B.1.1.529 (strain hCoV19/EHC_C19_2811C, green circles, n = 5) or B.1.351 (black triangles, n = 5). Far right: Weight change in 10 to 14-month-old C57BL/6 mice inoculated intranasally with 105 PFU of B.1.1.529 (strain hCoV19/EHC_C19_2811C, blue circles, n = 4), B.1.1.7 (black star, n = 10), or B.1.351 (black squares, n = 18). Data are mean ± SEM. b, Nasal wash and lung viral RNA levels in 129 mice inoculated with 104 (purple circles, n = 3) or 105 (blue circles, n = 3) FFU of B.1.1.529 (strain hCoV-19/USA/WI-WSLH-221686/2021) or 105 FFU of WA1/2020 N501Y/D614G (black circles, n = 3). c, Nasal turbinates and lung viral RNA levels in 129 mice inoculated with 106 PFU of B.1.1.529 (strain hCoV19/EHC_C19_2811C, green circles, n = 5) or B.1.351 (black triangles, n = 5) (** P < 0.01, by Mann-Whitney U test). d, Nasal turbinates and lung virus titers from BALB/c mice inoculated with 105 PFU of B.1.1.529 (strain hCoV-19/Japan/NC928–2N/2021, blue circles, n = 5) or B.1.351 (black squares, n = 5) (** P < 0.001, by Mann-Whitney U test). e, Pulmonary function analysis in infected BALB/c mice. Penh, a surrogate marker for bronchoconstriction or airway obstruction, was measured by whole body plethysmography. Data are presented as the mean ± SEM. P values were calculated by using pairwise comparisons after a linear mixed model analysis (***P < 0.001). Asterisks indicate statistically significant differences between B.1.351-infected (n = 5) and B.1.1.529-infected (n = 5) or uninfected animals (n = 5). f, Left: Weight change in 5-month-old K18-hACE2 transgenic mice inoculated intranasally with 103 (red circles, n = 3), 104 (purple circles, n = 6), or 105 (blue circles, n = 3) FFU of B.1.1.529 (strain hCoV-19/USA/WI-WSLH-221686/2021) or 103 FFU of WA1/2020 D614G (black triangles, n = 6). Right: Weight change in 6-month-old K18-hACE2 transgenic mice inoculated intranasally with 104 (purple circles, n = 6) PFU of B.1.1.529 (strain hCoV-19/USA/NY-MSHSPSP-PV44476/2021), or 104 PFU of B.1.351 (black hexagon, n = 6)). Age-matched uninfected mice (grey circles, n = 4) were included as controls. Data are presented as the mean ± SEM. g, Nasal wash and lung viral RNA levels in K18-hACE2 mice inoculated with 103 FFU of WA1/2020 D614G (black triangles, n = 3), or 103 (red circles, n =3) or 104 (purple circles, n =3) FFU of B.1.1.529 (strain hCoV-19/USA/WI-WSLH-221686/2021). The results are from one experiment and each symbol in b, c, d, and g represents an individual animal. The dotted line is the limit of detection. Infection studies in panels a, b and f with WA1/2020 N501Y/D614G are shown as comparisons and were adapted from published data22.