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. 2021 Dec 30;14:7431–7441. doi: 10.2147/JIR.S335293

Table 2.

mRNA Primer Sequences for RT-PCR

Genes Forward Primer (5ʹ-3ʹ) Reverse Primer (5ʹ-3ʹ) Product Length (bp) Temp (°C)
ABCC9 TCAACCTGGTCCCTCATGTCT CAGGAGAGCGAATGTAAGAATCC 155 59
AQP9 TGTCTCTTTGGACGGATGAAATG TCTCCCACGATCAGCAGTTTT 149 58
ARNTL2 TGGCGCGTAAACTGGACAAA CCTCTTTCACATCCAACCACAAA 187 59
CLDN1 CCTCCTGGGAGTGATAGCAAT GGCAACTAAAATAGCCAGACCT 145 57
EPB41L4B TTGGACCTGCTTATGCTTTACAC AGCTCCGCTTGTAGACAGAGA 184 58
IRAK3 CAGCCAGTCTGAGGTTATGTTT TTGGGAACCAACTTTCTTCACA 110 58
LIPG GATGGACGATGAGCGGTATCT CGCATCCGTGTAAAGCTGG 131 59
PCDH17 TCCTCGGACCAGATCGAAATG GCGGGACTTAACGTCCAGT 162 59
PDPN GTGTAACAGGCATTCGCATCG TGTGGCGCTTGGACTTTGT 80 59
PLAU CTGTCACCTACGTGTGTGGAG TGAGCGACCCAGGTAGACG 116 60
PPARGC1A GCTTTCTGGGTGGACTCAAGT GAGGGCAATCCGTCTTCATCC 175 60
SERPINE1 AGTGGACTTTTCAGAGGTGGA GCCGTTGAAGTAGAGGGCATT 151 58
SLC6A14 ACCGTGGTAACTGGTCCAAAA CGCCTCCACCATTGCTGTAG 114 58
SRD5A3 TGGCTGCACAGCTTACGAAG TCAGCACAGTTAGGCCAACAA 127 60
ZNF611 TAGGCCCCAAACCCAGATTTC TGCAAGGTATTGCTCGTGATT 232 58
GAPDH GCAAATTCCATGGCACCGTC AGCATCGCCCCACTTGATTT 110 59